CK-MB Type II possesses the naturally occurring carboxy-terminal amino acid lysine. This occurs during a myocardial infarct (MI or heart attack) when CK-MB Type II is released from damaged heart muscle, and the C-terminal lysine is cleaved in the blood stream, thus creating CK-MB Type I. This difference can be exploited in diagnosis of an MI.
Source:Recombinant protein corresponding to human CKMBITII, a glycosylated polypeptide chain having an identical aa sequence compared to the native enzyme, expressed in Pichia pastoris.
Biological Activity:Measured by the enzymatic activity of creatine phosphokinase procedure No.45-UV, 1IU-1umole creatine phosphate was 500IU/mg at 37°C.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Pichia pastoris
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl, pH 6.8, 1mM EDTA, 1mM DTT.
