Creatine Kinase BB is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. Reacts with polyclonal antibodies to BB isoenzyme in ELISA.
Source:Recombinant protein corresponding to human CKBBI, a glycosylated polypeptide chain with an identical aa sequence to the native enzyme, expressed in Pichia pastoris.
Biological Activity:Measured by the enzymatic activity of creatine phosphokinase procedure No.45-UV, 1IU-1umole creatine phosphate was 500 IU/mg at 37°C.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Pichia pastoris
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 10Mm Bis-Tris-HCl, pH 6.2, 0.5mM EDTA, 0.5mM DTT, 50% glycerol.
