Human chorionic gonadotropin (hCG) is a peptide hormone produced in pregnancy, that is made by the embryo soon after conception and later by the syncytiotrophoblast (part of the placenta). Its role is to prevent the disintegration of the corpus luteum of the ovary and thereby maintain progesterone production that is critical for a pregnancy in humans. hCG may have additional functions, for instance it is thought that it affects the immune tolerance of the pregnancy. Early pregnancy testing generally is based on the detection or measurement of hCG
Human Chorionic Gonadotropin Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, glycoprotein chain consisting of two non-covalently linked subunits - designated a and b consisting of 92 and 145 aa residues (237aa total, Mw: 36.7kD), respectively, with carbohydrate moieties linked to ASN-52 and ASN-78 (on a subunit) and ASN-13, ASN-30, SER-121, SER-127, SER-132 and SER- 138 (on b subunit). The primary structure of the a chain of r-hCG is identical to that of the a chain of hCG, FSH and LH. The glycoform pattern of the alpha subunit of r-hCG is closely comparable to urinary derived hCG (u-hCG), the differences mainly being due to the branching and sialylation extent of the oligosaccharides. The beta chain has both O and N-glycosylation sites and its structure and glycosylation pattern are also very similar to that of urinary hCG.
Source:Recombinant protein corresponding to human hCG, a single, glycoprotein chain consisting of two non-covalently linked subunits-designated a and b consisting of 92aa and 145aa residues (237aa total), expressed in CHO cells.
Molecular Weight:~36.7kD
Biological Activity:Determined by using the seminal vesicle weight gain test in male rats described in the "Chorionic Gonadotrophins" monograph of the European Pharmacopoeia. The in vivo biological activity of choriogonadotropin alfa has been calibrated against the third international reference preparation IS75/587 for chorionic gonadotropin.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：Highly Purified (~99%)
Form：Supplied as a lyophilized powder in 105mg sucrose, 3.43mg phosphoric acid. Reconstitute with ~100ug/ml sterile ddH2O.
