CDC34 is important in the control of cell cycle and DNA replication. Cdc34 in association with different E3 complexes, including SCF, has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. Cdc34 substrates that have been characterized include IkappaB, B-Myb, Wee1, MyoD, ICERIIgamma, ATF5, p27Xic1, and p27Kip1. Additionally, substrates such as beta-catenin, p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of Cdc34 by virtue of their SCF requirement for proteolysis. Cdc34 has been demonstrated to self-associate through a domain in the C-terminus, and is phosphorylated and ubiquitinylated in vivo. This protein is useful for in vitro ubiquitinylation reactions.
Source:Recombinant protein corresponding to human CDC34, a single, non-glycosylated polypeptide chain containing 248aa, fused to his-tag at N-terminal, expressed in E. coli.
Molecular Weight:~28kD
AA Sequence:MHHHHHHAMGILMARPLVPSSQKALLLELKGLQEE PVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYF KARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCIS ILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNE PNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQ VGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSD LFYDDYY EDGEVEEEADSCFGDDEDDSGTEES
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE)
Form：Supplied as a lyophilized powder in 1X PBS, pH 7.4, 1mM DTT. Reconstitute with ~100ul sterile ddH2O for ~1mg/ml stock.
