Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (aMb2) molecule, also known as macrophage-1 antgen (Mac-1) or complement receptor 3 (CR3). ITGAM is also known as CR3A, and cluster of differentiation molecule 11B (CD11B). The second chain of aMb2 is the common integrin beta2 subunit known as CD18, and integrin aMb2 thus belongs to the beta2 subfamily (or leukocyte) integrins. aMb2 is expressed on the surface of many leukocytes involved in the innate immune system, including monocytes, granulocytes, macrophages, and natural killer cells. It mediates inflammation by regulating leukocyte adhesion and migration and has been implicated in several immune processes such as phagocytosis, cell-mediated cytotoxicity, chemotaxis and cellular activation. It is involved in the complement system due to its capacity to bind inactivated complement component 3b (iC3b). The ITGAM (alpha) subunit of integrin aMb2 is directly involved in causing the adhesion and spreading of cells but cannot mediate cellular migration without the presence of the beta2 (CD18) subunit. In genome wide association studies, single nucleotide polymorphisms in ITGAM had the strongest association with systemic lupus erythematosus, with an odds ratio of 1.65 for the T allele of rs9888739 and lupus.
Source:Recombinant protein corresponding to aa936-1154 from human CD11b, expressed in E. coli.
Molecular Weight:~52kD
Applications:Suitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in 50mM Tris-acetate, pH 7.5, 1mM EDTA, 20% glycerol.
