The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinase that is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathway activation. A Pertussis toxin-sensitive G protein and Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2. Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm.
Source:Recombinant protein corresponding to human Casein Kinase 2 beta, a non-glycosylated polypeptide, expressed in E. coli.
Molecular Weight:~24.9kD
Specific Activity:~1U/mg
Unit Definition:1U=1umol/min at 37°C using the synthetic peptide RRRDDDSDDD.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O or 50% glycerol. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：3ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in 25mM Tris-HCl, pH 8.5, 500mM sodium chloride, 1mM DTT, 1mM EDTA, protease inhibitor cocktail. Reconstitute with sterile ddH2O or 50% glycerol.
