The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinase that is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathway activation. A Pertussis toxin-sensitive G protein and Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.
Source:Recombinant protein corresponding to Zea Mays Casein Kinase 2 alpha, a non-glycosylated polypeptide, expressed in E. coli.
Molecular Weight:~39.2kD
Specific Activity:~1U/mg
Unit Definition:1U=1umol/min at 37°C using the synthetic peptide RRRDDDSDDD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
