The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinase that is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathway activation. A Pertussis toxin-sensitive G protein and Disheveled appear to be an intermediary between Wnt-mediated activation of theFrizzled receptor and activation of casein kinase 2. Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm.
Source:Recombinant protein corresponding to human Casein Kinase 2 alpha, a non-glycosylated polypeptide, expressed in E. coli.
Molecular Weight:~45.1kD
Specific Activity:~1.320U/mg
Unit Definition:1U=1umol/min at 37°C using the synthetic peptide RRRDDDSDDD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：3ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~99% (SDS-PAGE)
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.5, 500mM sodium chloride, 1mM DTT, 500uM PMSF, 50% glycerol.
