Myc gene was first discovered in Burkitt's lymphoma patients. In Burkitt's lymphoma, cancer cells show chromosomal translocations, in which Chromosome 8 is frequently involved. Cloning the break point of the fusion chromosomes revealed a gene that was similar to myelocytomatosis viral oncogene (v-Myc). The new cellular gene was named c-Myc. Human c-myc (accession # P01106; 439aa, chromosome 8q24) is a protooncogene, which is overexpressed in a wide range of human cancers. When it is specifically-mutated, or overexpressed, it increases cell proliferation and functions as an oncogene. It is a transcription factor that regulates expression of a great number of genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc belongs to Myc family of transcription factors, which also includes N-Myc and L- Myc genes. Myc-family transcription factors contain the bHLH/LZ (basic Helix-Loop-Helix Leucine Zipper) domain.
An epitope located within aa410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tag fusion proteins. Epitope tags antibodies provide a method to immunolocalize the myc-fusion gene products in a variety of cell types, to study the topology of proteins and protein complexes, and to identify associated proteins. In addition, they allow characterization of newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. It is also possible to use ant-tag antibodies for the purification of fusion proteins. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Source:Recombinant protein corresponding to full-length human C-myc, expressed as fusion protein (T7-tag-His-tag- Myc) in E. coli.
Molecular Weight:~65kD
Applications:Suitable for use in ELISA and as a Positive Control for Western Blot. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in 50mM Tris, pH 8.0, 250mM sodium chloride, 0.75% sarcosine, 10mM beta mercaptoethanol.
