The BMPs belong to the TGF-Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and–II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.
Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2,-4,-5,-7,-14 (CDMP-1/GDF-5), other members, such as BMP-3,-6,-7 and -13 (CDMP-2/GDF-6) may be involved in later stages of skeletal formation.
BMP7 or OP1a is a 431aa polypeptide that includes a secretory signal sequence, (Chr 20), expressed in kidney, bladder and brain. Incuces cartilage and bone formation may be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. It plays a role in calcium regulation and bone homeostasis.
Source:Recombinant protein corresponding to mature human BMP7, as a monomeric, non-glycosylated protein of 139aa, expressed in E. coli.
Molecular Weight:~15.6kD
Endotoxin:<0.1ng/1ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a lyophilized powder in 0.1% BSA. Reconstitute with sterile 40mM acetate, pH 5.0, 0.1% HSA or BSA.
