The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and –II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.
Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1 / GDF-5), other members, such as BMP-3, -6, -7 and –13 (CDMP- 2 / GDF-6) may be involved in later stages of skeletal formation.
BMP6 or VGR1, a 57kD protein with 513aa in human (chr 6p24), Increased production of BMP6 is mediated by the skeletal effects of estrogen on bone and cartilage, BMP-6 differs from other members of the BMP family by its concentration in cartilage of the fetus.
Source:Recombinant protein corresponding to human BMP-6, expressed in mouse myeloma cell line, NSO.
Molecular Weight:~15kD, due to glycosylation the rhBMP-5 migrates as an ~36kD protein under non-reducing conditions and as doublet of 18kD and 23kD protein under reducing conditions in SDS-PAGE.
Biological Activity:Determined by its ability to inducealkaline Phosphatase production by A TDC5 chondrogenic cells (ED50=0.05-0.15ng/ml).
Endotoxin:<1.0ng/1ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
