The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and –II receptors. The primary unit of bone formation is osteoblast, the boneforming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.
Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1 / GDF-5), other members, such as BMP-3, -6, -7 and –13 (CDMP-2 / GDF-6) may be involved in later stages of skeletal formation.
BMP14/CDMP-1/ GDF-5, a 501aa precursor protein with a 121aa mature chain, (chromosome 20q11.2). It is predominantly expressed in long bones during embryonic development; it is involved in bone formation. Defects in GDF5/CDMP-1 are a cause of Acromersomelic chondrodysplasia; short forearms, hands and feet, characterize this form of dwarfism.
Source:Recombinant protein corresponding to 121aa from human BMP-14/CDMP-1(13.5kD homodimeric disulfide-linked protein), expressed in E. coli.
Molecular Weight:~13.5kD
Biological Activity:Determined by its ability to induce alkaline phosphatase production by ATDC5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0ug/ml.
Endotoxin:<0.1ng/1ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~98%)
Form：Supplied as a lyophilized powder in BSA. Reconstitute with sterile ddH2O containing BSA.
