A1AG serum protein, also called orosomucoid, is a monomer about 210aa residues long; the amino acid sequence has been determined through 192aa. A1AG is found in feline plasma in concentrations of 55-140mg per 100ml. It is a classical standard glycoprotein (40kD, ~40% carbohydrates) for studies on the structure of the oligosaccharide units. Its biological significance is unknown, although it can bind progesterone 15x as strongly as albumin. Sialic- acid-deficient alpha-1-AG has an affinity for vitamin B-12. Clinically, alpha 1 acid glycoprotein is an acute-phase reactant that together with haptoglobin is an indicator of acute inflammation. The alpha 1 acid glycoprotein:haptolobin ratio is useful in studies of bone marrow disorders, hemolytic processes and metastases. It is a positive acute phase reactant, the levels of which increase significantly in cats in response to inflammation, tissue injury and disease. A1AGP is purified using a combination of acid extraction, precipitation, gel filtration and affinity chromatography steps.
Source:A1-AGP, from bovine plasma
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Bovine plasma
Grade：Highly Purified
Purity：Highly Purified (~99%)
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
