Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108kD protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.
Source:Recombinant protein corresponding to human Alanyl t-RNA Synthetase, fused to 6x his-tag, expressed in Sf9 insect cells.
Molecular Weight:~107.6kD
Immunological Functions:Binds IgG-type human auto-antibodies.
Applications:Suitable for use in standard ELISA (checker-board analysis of positive/negative sera panels immuno-dot test), Western Blot (with monoclonal anti-hexa-His-tag antibody & Polymyositis sera), Biotinylation and Iodination. Other applications not tested.
Recommended Dilution:ELISA: 0.3-0.8ug/ml (depending on the type of ELISA plate and coating buffer)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：5ug
Source Antigen：Recombinant, sf9 insect cells
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in 16mM HEPES, pH 8, 250mM sodium chloride, 20% glycerol.
