Akt1, also known as "Akt" or protein kinase B (PKB) is an important molecule in mammalian cellular signaling. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. These enzymes are members of the serine/threonine-specific protein kinase family (EC2.7.11.1). Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogenein the transforming retrovirus, AKT8.
Source:Recombinant protein corresponding to human AKT1, expressed in Sf9 insect cells.
Molecular Weight:~59.1kD
Biological Activity:~20,000Units/mg
Unit Definition:1 Unit=1 pmol/min transferred to synthetic peptide RPRAATF at 30ºC. No protease activity detectable.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, sf9 insect cells
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in 50mM sodium chloride, pH 8.5, 1mM DTT, 25mM beta glycerophosphate, 50% glycerol.
