IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant human IL-7 is a 17.4kD protein containing 153aa residues. Manufactured using all non-animal reagents.
Recombinant protein corresponding to human IL-7 expressed in E. coli.
Synonyms:Lymphopoietin; 1LP-1; Pre-B Cell Factor
Appearance:Supplied as a lyophilized powder. No additives. Reconstitute with ddH2O. Do not vortex.
Purity:≥98% (SDS-PAGE, HPLC)
Endotoxin:As reported
Biological Activity:Determined by its ability to stimulate the proliferation of mouse 2E8 cells. The expected ED50 is ≤0.5ng/ml, corresponding to a specific activity of ≥2x1e6units/mg.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Cell Culture Grade
Purity：≥98% (SDS-PAGE, HPLC) Endotoxin: ≤0.1ng/ug of protein (≤1EU/ug)
Form：Supplied as a lyophilized powder. No additives. Reconstitute with ddH2O. Do not vortex.
