85-2063-01　IL-35, Recombinant, Mouse, Fc: Human (Interleukin-35) 5ug　208531

特徴

• Interleukin-35 (IL-35) is a novel IL-12 family cytokine produced by regulatory T cells (Treg) but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of EBI3 (Epstein-Barr-Virus-induced gene 3) and IL-12a (p35). EBI3 is a downstream target of Foxp3, a transcription factor required for Treg-cell development and function, and thus Treg-cell restriction of IL35 occurs. Regulatory T cells are essential for maintaining self tolerance and preventing autoimmunity, and IL-35 is identified as a molecule that mediates the immune suppression function of Treg-cell. As an inhibitory cytokine, IL-35 induces proliferation of Treg-cell populations but suppresses Th17 cell development. Studies in mice show the absence of either IL-35 chain from Treg-cell reduces the cells' ability to suppress inflammation using an experimental model for inflammatory bowel disease. IL-35 is suggested as a potential target of immunotherapy.
• Source:Recombinant protein corresponding to aa22-228 from mouse IL-35 complex composed of the Ebi3 subunit, and aa23-215 from IL-12a (p35) subunit, fused through a polypeptide linker to the Fc region of human IgG1, expressed in CHO cells.
• Biological Activity:Bioactivity was measured in a cell proliferation assay of Con A activated mouse splenocytes.
• Endotoxin:<0.06EU/ug (LAL test, Lonza)
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.