Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta1 (TGF-beta1) and bone morphogenetic protein 2 (BMP-2). Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. Periostin up-regulation in cancers usually correlates with aggressiveness and/or poor survival.
The mouse Periostin BioAssay™ ELISA Kit is to be used for the in vitro quantitative determination of mouse periostin in cell culture supernatants, serum and plasma.
Sensitivity:10pg/ml
Range:31-2000pg/ml
Specificity:Recognizes natural and recombinant mouse periostin. Has been tested on mouse periostin isoform 5 (should also detect mouse isoforms 1, 2 and 3 according to sequences). Mouse isoform 4 has not been tested.
Test Principle:This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of periostin in the samples.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
