PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Source:Recombinant protein corresponding to full-length human PARP-1, fused to MYC and His-tag, expressed in Sf21 cells.
Biological Activity:≥1200U/mg protein
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Sf21 cells.
Grade：Highly Purified
Purity：≥98% (SDS-PAGE)
Form：Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 50mM imidazole, 0.2% NP-40, 10% glycerol.
