85-2061-28　［取扱停止］PARP1, Catalytic domain, Recombinant, Human, aa656-1014, His-Tag (Poly [ADP-ribose] Polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] Synthase 1, ADPRT, PPOL) 20ug　207327

特徴

• PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
• Source:Recombinant protein corresponding to aa656-1014 from human PARP-1, fused to MYC and His-tag, expressed in Sf21 cells.
• Biological Activity:~50ng are required for poly(ADP-ribose) formation.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.