Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
Source:Recombinant protein corresponding to human PARG, fused to His-tag, expressed in Sf21 cells. Swiss/UniProt Accession: Q86W56.
Biological Activity:~100ng is required for poly(ADP-ribose) degradation assays
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2ug
Source Antigen：Recombinant, Sf21 cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.2% NP-40, 50mM imidazole, 10% glycerol
