85-2061-13　NMNAT1, Recombinant, Human, aa1-279, His-Tag, Highly Active (Nicotinamide Mononucleotide Adenylyltransferase 1, NMN Adenylyltransferase 1, Nicotinate-nucleotide Adenylyltransferase 1, NaMN Adenylyltransferase 1, NMNAT, EC 2.7.7.1) 50ug　207304

特徴

• Nuclear NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. It can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Interacts with PARP-1/ARTD1. Protects against axonal degeneration following mechanical or toxic insults. Widely expressed.
• Recombinant protein corresponding to full-length NMNAT1, fused to His-tag at N-terminal, expressed in E. coli.
• Molecular Weight:~33.3kD (monomer)
• Applications:Well suited for the synthesis of NAD due to high specific activity and high substrate selectivity compared to NMNAT3. For NAD analog synthesis use NMNAT3. When loss of activity is observed add 10mM DTT to the working buffer and incubate for at least 15 min before the assay.
• Biological Activity:≥5U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol of NAD+ per min.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.