NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside).
Source:Recombinant protein corresponding to full-length Haemophilus influenzae NAD Nucleotidase, without the signal peptide corresponding to the first 26aa, expressed in E. coli.
Molecular Weight:~63kD
Biological Activity:With AMP: 856.4nmol of Ade/min/mg of proteinWith NMN: 110,3nmol of NR/min/mg of proteinWith NAD: 5.4nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE)
Form：Supplied as a liquid in 5mM KH2PO4, pH 6.8, 100mM TRIS-HCl, pH 8.5, 10mM magnesium chloride.
