NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target.
Catalytic activity:ATP + NAD+ = ADP + NADP+
Source:Recombinant protein corresponding to Mycobacterium tuberculosis NAD kinase, fused to His-tag at N-terminal, expressed in E. coli.
Molecular Weight:~33kD
Biological Activity:~1.2 U/mg protein. (Substrate: NAD (+ ATP))~3.8 U/mg protein. (Substrate: NAD (+ poly(P))One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE)
Form：Supplied as a liquid in 10mM TRIS, pH 8.0, 1mM MgCl2, 0.5mM EDTA, 1mM DTT, 1mM PMSF.
