GCN5 functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. It has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. In case of HIV-1 infection, it is recruited by the viral protein Tat. It regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. GCN5 is a component of the Ada Two-A containing (ATAC) complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Source:Recombinant protein corresponding to human GCN5, fused to a His-tag, expressed in Sf21 cells.
Biological Activity:~200-300ng are required for standard HAT assay
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2ug
Source Antigen：Recombinant, Sf21 cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 0.2% NP-40, 50mM imidazole, 10% glycerol.
