85-2059-97　FLT3 Ligand, Recombinant, Human (FMS-like Tyrosine Kinase 3 Ligand) 10ug　207185

特徴

• FMS-like tyrosine kinase 3 ligand (FLT3L) acts as a growth factor that increases the number of immune cells (lymphocytes (B cells and T cells)) by activating the hematopoietic progenitors. It binds to FLT3 (CD135) which is found on multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells in mice. FLT3L induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3L is crucial for steady-state pDC (plasmacytoid dendritic cells) and cDC (classical dendritic cells) development. Deficiency of FLT3L causes a dramatic decrease in DC numbers, whereas increasing its availability increase in DC numbers.
• Source:Recombinant protein corresponding to the receptor-binding domain of human FLT3L, fused at N-terminal to the Fc portion of human IgG1, expressed in CHO cells.
• Biological Activity:In vitro and in vivo generation of conventional and plasmacytoid dendritic cells (DC). FLT3L increases in vivo both NK and DC cells numbers in the spleen when mice are injected intraperitoneally with 2 or 10ug, daily for 10 days (slightly higher effect at 10ug).
• Endotoxin:<0.1EU/ug purified protein (LAL test; Lonza)
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.