Human B7-H2 is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Source:Recombinant protein corresponding to aa1-256 from human CD275, fused to the N-terminus of the Fc region of mouse IgG2a, expressed in CHO cells.
Biological Activity:Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3.
Endotoxin:~0.06EU/ug protein (LAL test; Lonza)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：~98% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 100ug/ml.
