CD134 (OX40) is a T cell activation antigen structurally belonging to a lymphocyte-specific subgroup of the nerve growth factor and tumor necrosis factor receptor superfamily, which also includes the T cell antigen CD27, B cell antigen CD40, FAS antigen and the T cell activation antigen 4-1BB. The human CD134 protein is expressed only on activated CD4+ T blasts, and its ligand has been identified as gp34. Interactions between CD134 and its ligand in vivo are necessary for the differentiation of activated B cells into highly immunoglobulin-producing cells, however not involved in other pathways of antigen-driven differentiation of B cells such as development of memory cells in the germinal centers. In addition, the CD134 and gp34 system directly mediate adhesion of activated T cells to vascular endothelial cells, and contribute to growth stimulation of the virus-infected T cells.
Source:Recombinant protein corresponding to aa29-216 from human CD134, fused to the N-terminus of the Fc region of human IgG1, expressed in CHO cells.
Biological Activity:Measured by its binding ability in a functional ELISA.
Endotoxin:~0.06EU/ug protein (LAL test; Lonza)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：≥98% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 50ul sterile ddH2O. Add 1X PBS to the desired protein concentration.
