85-2057-83　GroEL, Recombinant (Chaperonin 60, cpn60, Heat Shock Protein 60, HSP60) 100ug　G8976-01A1

特徴

• GroEL protein is the major heat shock protein of E.coli and belongs to the chaperonin(HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques. Recombinant GroEL produced in E. coli is a single,non-glycosylated polypeptide chain conjtaining 548 amino acids and having a molecular mass of 57.3kD.
• Source:Recombinant, E. coli
• Synonyms:Chaperonin 60; cpn60; Heat Shock Protein 60; HSP60
• Molecular Weight:57.3kD
• Appearance:Supplied as a liquid in 50mM Tris buffer, pH 7.5, 150mM potassium chloride, 10mM magnesium chloride, 1mM DTT, 2.5% trehalose.
• Purity:≥95% (SDS-PAGE)
• Concentration:~1mg/ml
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.