Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications.
Applications:Suitable for use in DNA labeling by PCR with taq polymerase, DNA labeling by nick translation with DNase I, DNA labeling by primer extention with Klenow 3’-5’ exo-, 3’ end DNA labeling with terminal deoxynucleotidyl transferase (TdT), cDNA labeling by reverse transcription with Moloney Murine Leukemia Virus Reverse Transcriptase. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1ml
Grade：Highly Purified
Purity：≥ 95% (by HPLC)
Form：Supplied as a sterile liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin.
