85-2057-57　［取扱停止］Mast Cell Tryptase (MCT) BioAssay™ ELISA Kit (Monkey) 48Tests　196452

特徴

• The Mast Cell Tryptase (MCT) BioAssay™ ELISA Kit is a 1.5-hour solid-phase competitive ELISA designed for the quantitative determination of MCT in monkey serum, plasma, tissue homogenates, cell culture supernates and body fluids. This kit is intended for research use only and may not be used for therapeutic or diagnostic applications.
• Detection Range:2.5-50ng/ml
• Sensitivity:0.1ng/ml
• Precision:Intra-assay %CV: <10Inter-assay %CV: <10
• Specificity:This assay has high sensitivity and excellent specificity for detection of MCT. No significant crossreactivity or interference with analogs was observed. Note: Crossreactivity detection between the MCT and all analogs has not been determined and crossreaction may exist in some cases.
• Kit Components:196452A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated with anti-MCT antibody196452B: Enzyme Conjugate, 1x3ml or 1x6ml196452C: Standard, 0ng/ml, 1x1 vial196452D: Standard, 2.5ng/ml, 1x1 vial196452E: Standard, 5ng/ml, 1x1 vial196452F: Standard, 10ng/ml, 1x1 vial196452G: Standard, 25ng/ml, 1x1 vial196452H: Standard, 50ng/ml, 1x1 vial196452J: Substrate A, 1x6ml196452K: Substrate B, 1x6ml196452L: Stop Solution, 1x6ml196452M: Wash Solution, 100X, 1x10ml196452N: Balance Solution, 1x3ml
• Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Assay Principle:The Mast Cell Tryptase (MCT) BioAssay™ ELISA Kit (Monkey) employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate that is has been pre-coated with a monoclonal anti-MCT antibody. The assay samples and standards are incubated together with MCT-HRP conjugate for one hour. A competition for limited antibody binding sites on the plate occurs between MCT-HRP conjugate and MCT in the samples and standards. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to terminate the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCT concentration in the sample or standard. A standard curve is plotted relating the intensity of the color (O.D.) to the concentrations of the standards. The MCT concentration in each sample is interpolated from this standard curve.
• Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.net
• Tech Support: [email protected] Service: [email protected]: 781-639-5092 • 800-520-3011Fax: 978-594-8052