85-2057-55　［取扱停止］Dentin Sialoprotein (DSP) BioAssay™ ELISA Kit (Human) 48Tests　190989

特徴

• Dentin Sialoprotein (DSP) BioAssay™ ELISA Kit is a 1.5 hour solid-phase Competitive ELISA designed for the quantitative determination of DSP in human serum, plasma, cell culture supernatants, body fluids and tissue homogenates. This kit is intended for research use only and may not be used for therapeutic or diagnostic applications.
• Detection Range:0.5-10ng/ml
• Sensitivity:0.1ng/ml
• Precision:Intra-Assay %CV: <10Inter-Assay %CV: <10
• Specificity:This assay has high sensitivity and excellent specificity for detection of DSP (Human) No significant crossreactivity or interference with analogs was observed
• Note: Crossreactivity detection between the DSP and all analogs has not been determined and crossreaction may exist in some cases.
• Kit Components:190989A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated with anti-DSP antibody190989B: Enzyme Conjugate, 1x3ml or 1x6ml190989C: Standard, 0ng/ml, 1x1 vial190989D: Standard, 0.5ng/ml, 1x1 vial190989E: Standard, 1ng/ml, 1x1 vial190989F: Standard, 2.5ng/ml, 1x1 vial190989G: Standard, 5ng/ml, 1x1 vial190989H: Standard, 10ng/ml, 1x1 vial190989J: Substrate A, 1x6ml190989K: Substrate B, 1x6ml190989L: Stop Solution, 1x6ml190989M: Wash Solution, 100X, 1x10ml190989N: Balance Solution, 1x3ml
• Note:The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagent. 
• Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Assay Principle:The Dentin Sialoprotein (DSP) BioAssay™ ELISA Kit (Human) employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate that has been pre-coated with an anti-DSP antibody. The assay samples and standards are incubated together with DSP-HRP conjugate for one hour. A competition for limited antibody binding sites on the plate occurs between DSP-HRP conjugate and DSP in the samples and standards. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to terminate the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DSP concentration in the sample or standard. A standard curve is plotted relating the intensity of the color (O.D.) to the concentrations of the standards. The DSP concentration in each sample is interpolated from this standard curve.
• Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.net
• Tech Support: [email protected] Service: [email protected]: 781-639-5092 • 800-520-3011Fax: 978-594-8052