Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerousapplications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring. Simple, direct and automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular. The phosphate assay kit is designed to measure phosphate ion directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Intended Use:For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism.
Applications:Direct Assays: Pi in serum, urine, saliva, sweat, tissue culture etc
Drug Discovery/Pharmacology: effects of drugs on Pi metabolism
Food and Beverages: Pi determination
Environment: Pi determination in water, soil and fertilizer.
Range:0.30um (0.0028mg/dL)-50um (0.47mg/dL) phosphate in 96-well plate assay.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
