Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate. The Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570nm orfluorescence intensity at lex/em = 530/585nm is directly proportional to the oxaloacetate concentration in the sample.
Intended Use:For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism.
Applications:Direct Assays: oxaloacetate in plasma, serum, tissue and culture media.
Range:7-400um oxaloacetate for colorimetric assays and 1-40um for fluorometric assays.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
