The Malachite Green Phosphate BioAssay™ Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be executed in tubes, cuvettes or multi-well plates. The assays can be conveniently performed in 96- and 384-well plates for high-throughput screening of enzyme inhibitors.
Intended Use:For sensitive and high-throughput phosphate determination.
Applications:Phosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate
Lipase Assays: liberation of phosphate from phospholipids Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc). Quantitation of Phosphate in phospholipids, proteins and DNAs, etc
Drug Discovery: high-throughput screen for phosphatase inhibitors.
Range:0.02um-40um phosphate.
Detection limit:0.02um
Kit Components:Reagent A, 1x50mlReagent B, 1x1ml Standard, 1x1ml 1mM phosphate
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
