Isocitrate (Isocitrate Acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonlyfound in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. The isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highlyfluorescent product. The fluorescence intensity of this product, measured at lex/em= 530/585nm, is proportional to the isocitrate concentration in the sample.
Intended Use:For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
