Glutamate Dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and a-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.
This non-radioactive, colorimetric GLDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565nm. The increase in absorbance at 565nm is directly proportional to the enzyme activity.
Intended Use:For quantitative determination of glutamate dehydrogenase activity in plasma, serum, urine, tissue and culture media.
Storage and Stability:Store all components at -20°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
