Glucose oxidase catalyzes the oxidation of glucose from D-glucose to Dglucono-d-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct and high throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery. The glucose oxidase assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at lex/em=530/585nm is directly proportional to glucose oxidase activity in the sample.
Intended Use:For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.
Applications:Direct Assays: glucose oxidase activity in cell lysate, culture medium and other biological samples
Drug Discovery/Pharmacology: effects of drugs on glucose metabolism
Range:0.02-10U/L glucose oxidase for colorimetric assays and 0.002-1.5U/L for fluorometric assays.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
