Glucose (C6H12O6) is a ubiquitous fuel molecule in biology. It is oxidized through a series of enzyme-catalyzed reactions to form carbon dioxide and water, yielding the universal energy molecule ATP. Due to its importance in metabolism, glucose level is a key diagnostic parameterfor many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and automation-ready procedures for measuring glucose concentrations find wide applications in research and drug discovery. The glucose assay kit is designed to measure glucose directly in serum or plasma without any pretreatment. The improved otoluidine method utilizes a specific color reaction with glucose. The absorbance at 630nm is directly proportional to glucose concentration in the sample.
Intended Use:For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
Applications:Direct Assays: glucose in biological samples (e.g. serum and plasma)
Drug Discovery/Pharmacology: effects of drugs on glucose metabolism
Food and Beverages: glucose in food, beverages etc.
Range:0.7mg/dL (39uM)-300mg/dL (16.6mM) glucose in 96-well plate.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
