Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570nm or fluorescence intensity at lex/em=530/585nm is directly proportional to the citrate concentration in the sample.
Intended Use:For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.
Applications:Citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
Range:4-400um citrate for colorimetric assays and 0.5-40um for fluorometric assays.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
