Human TEK tyrosine kinase, endothelial, also known as Tie2, (GenBank Accession No. NM_000459) a.a.771-1124(end) with N-terminal GST tag, MW= 66kD, expressed in baculovirus infected Sf9 cell expression system.
Source:Recombinant protein corresponding to aa771-1124 from human Tie2 at N-terminal, fused to GST tag at N-terminal. expressed in baculovirus infected Sf9 cell expression system.
Molecular Weight:~66kD
Specific Activity:1400 pmol/min/ug
Assay Condition:Enzyme reaction is conducted in a buffer containing 50mM HEPES (pH 7.5), 10mM MgCl2, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate (Tyr Peptide 5, Z-lyte kinase assay kit from Invitrogen), and enzyme at 37°C for 1 hr.
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：~80%
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT, 20% glycerol.
