The SRE Reporter BioAssay™ Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency.
Applications:Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity
Screen for activators or inhibitors of the MAPK/ERK signaling pathway
Study effects of RNAi or gene overexpression on the activity of the MAPK/ERK pathway.
Components:Reporter (Component A), 1x500ulNegative Control Reporter (Component B), 1x500ul
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
