Renin is a protease that is synthesized by the kidneys. Renin catalyzes the hydrolysis of angiotensinogen to angiotensin I, which is converted to angiotensin II, a highly potent vasoconstrictor. Renin inhibitors have been used therapeutically in the treatment of hypertension (high blood pressure).
The Renin BioAssay™ Kit is designed to measure Renin activity for screening and profiling applications. It comes in a convenient 96-well format, with purified, activated renin, renin substrate, and assay buffer for 100 enzyme reactions. Aliskiren Hydrochloride is also included as a control for renin inhibition. The key to the Renin Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for renin reactions. The fluorometric substrate is incubated with a sample containing renin enzyme to produce a fluorophore that can then be measured using a fluorescence reader. Note: This kit is also suitable for use with cell extracts.
Applications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
