Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a25-167, with C-terminal expressed in a HEK293 cell expression system and enzymatically biotin-labeled. MW = 44.5kD. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Source:Recombinant protein corresponding to aa25-167 from PD-1 at C-terminus, expressed in a HEK293 cell expression system.
Molecular Weight:~44.5kD
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectively profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Amino Acid Sequence:(25)LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAA FPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESL RAELRVTERRAEVPTAHPSPSPRPAGQFQ (167)IEGRMD (linker)(100)PKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGVFSCSVMHEALHNHYTQKSLSLSPGK (330) Fc of human IgG1GGGLNDIFEAQKIEWHE (Avi-tagTM)
仕様
Size：50ug
Source Antigen：Recombinant, HEK293
Grade：Purified
Purity：As reported
Form：Supplied as a liquid in 8mM phosphate, pH 7.4 110mM NaCl, 2.2mM KCl, 20% glycerol. Labeled with Biotin.
