The PCSK9 [Biotinylated]-LDLR Binding BioAssay™ Kit is designed for screening and profiling purposes. PCSK9 is known to function as a negative regulator of hepatic low-density lipoprotein receptors (LDLRs) by binding to the LDLR ectodomain. The PCSK9[Biotinylated]-LDLR Binding Assay Kit comes in a convenient 96-well format, with biotin-labeled PCSK9, purified LDLR ectodomain, streptavidin labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Applications:Great for screening small molecules and antibodies that inhiit the binding of PCSK9 to LDLR ectodomain.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
