Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a.2-130(end)), H2B (a.a.2-126(end)), H3 (a.a.2-137(end)), and H4 (a.a.2-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Total histone MW=113.8kD.
Source:Recombinant protein corresponding to aa2-137 from histone proteins at the N-Terminus, expressed in E.coli.
Molecular Weight:~113.8kD
Application:Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~99%
Form：Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM EDTA, 2mM DTT, 20% glycerol.
