Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a1-979 (end), delta 346-731, with N-terminal GST tag, MW =92kD, expressed in Sf9 insect cells via a baculovirus expression system
Source:Recombinant protein corresponding to human NEK9 at N-terminal, fused to GST-tag expressed in Sf9 insect cells via a baculovirus expression system.
Molecular Weight:~92kD
Specific Activity:147pmol/min/ug
Assay Buffer:Enzyme reaction is conducted in a Kinase Buffer (50mM Tris pH 7.4, 20mM MgCl2, 0.1mg/ml BSA and 1mM DTT) containing 20 CM ATP and 0.2mg/ml MBP for 30 minutes at 30oC.
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：Purified (~38%)
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8, 110mM sodium chloride, 2.2mM potassium chloride, 4mM Glutathione, 3mM DTT, 20% glycerol.
