Recombinant human NEK7, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Source:Full length recombinant protein corresponding to human NEK7 at N-terminal, fused to GST-tag expressed in Sf9 insect cells.
Molecular Weight:~63kD
Specific Activity:47pmol/min/ug
Assay Conditions:Assay was done in a reaction buffer (25ul) containing 40mM Tris, pH 7.4, 20mM MgCl2, 0.1mg/ml BSA, 100uM ATP, 0.2mg/ml Casein and NEK7 for 50 min at 30 oC.
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：Purified (~72%)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
