Recombinant human MEKK2, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Source:Full length recombinant protein corresponding to human MEKK2 at N-terminal, fused to GST-tag expressed in Sf9 insect cells.
Molecular Weight:~96.5kD
Specific Activity:18pmol/min/ug
Assay Conditions:Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 uM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C.
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:10ug
Source Antigen:Recombinant, baculovirus
Grade:Purified
Purity:Purified (~87%)
Form:Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 20% glycerol.