The Kinetic HDAC3 BioAssay™ Kit is a complete assay system designed to measure histone deacetylase 3 (HDAC3) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified HDAC3/NCOR2 enzyme complex and a potent HDAC inhibitor, SAHA, for use as a positive control. The Kinetic HDAC3 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC3 reaction on a microtiter plate is needed and then the HDAC3 activity level can be measured kinetically using a fluorescence reader.
Applications:Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Components:HDAC3/NCOR2 human recombinant enzyme, 1x2ugFluorogenic HDAC substrate (5mM), 1x50ul10x HDAC Developer, 1x1mlDeveloper Dilution Buffer, 1x10mlSAHA (1mM), 1x20ulHDAC Assay BUffer, 1x20mlBSA (1mM), 1x1mlblack, low binding NUNC black mictotiter plate, 1x1plate
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
